Chalcone Against Interferon Gamma (IFN-γ) Induced Oxidative Stress Indicate by Nitric Oxide Production
Abstract
Interferon (IFN)-γconfers crucial immune surveillancepositively for immune-modulation, one of which is as antimicrobial such as antiviral replication, microbial killing, and MHC induction. Gamma interferon (IFN-γ) also a critical cytokine in host defense against salmonella infections. IFN-γ activation renders the macrophages capable of killingintracellular mycobacteria by overcoming the phagosome maturation block, nutrient deprivation and exposure tomicrobicidal effectors including nitric oxide (NO).Chalcones have been linked with immunomodulation, antibacterial, antifungal, antiviral, antiinflammatory,antioxidant, anticancer, and antidiabetic activities. In this study, we aim to prove this speculation whether Chalcone can reduce oxidative inflammatory responses by targeting Nitric Oxide production.Changes in cell viability and cytotoxicity in murine macrophage RAW264.7 cells treated with hydrogen peroxide and chalcones were monitored.By using the sub-lethal dosages of these treatments, IFN-γ-induced the production of Nitric Oxide used as biomarker of oxidative stress. These results indicate that chalcone confers antioxidant activity against oxidative stress indicate by Nitric oxide production