THE ACTIVITY TEST OF PANDAN WANGI LEAVES EXTRACT (PANDANUS AMARYLLIFOLIUS ROXB) FOR SOLUBILITY OF CALSIUM KIDNEY STONES BY IN VITRO METHOD USING ATOMIC ABSORBTION SPECTROPHOTOMETRI (AAS)

Abstract

Pandanus amarylliflius Roxb.folium has been used from generation to generation as kidney stones medicine. Mursito (2001) in his book said thaPandanus amarylliflius Roxb folius has flavonoid as one of the constituents. In accordance with  Pramono (1986), one of the kidney stones medicine mechanisme is by forming dissolvable calcium-flavonoid complex. The experiment was purposed to know whether water extract of  Pandanus amarylliflius Roxb folium contains flavonoid and could dissolve calcium contained in kidney stones. Kidney stones as subject were homogenized its fitrated sefted using a mess 45, and then to test of qualitatively analized chemically. Pandanus amarylliflius  extracted with water and get analized qualitatively with Wilstatter Test. The kidney stones divided into seven group, each of them soaked in aquadest, Calcusol 1,2%, solution, Pandanus amarylliflius Roxb. Folium extract 0,625%, 1,25%, 2,5%, 5% and 10%,during ±5 hour with temperature ±37°C. Quantitative analize with Atomic Absorption Spectrophotometer was done to the solution after soaking. Qualitative analysis of kidney stones calcium shows positive results. Flavonoid qualitatif analysis with Wilstatter Test shows possitive results of flavonoid with carmine colours. Atomic Absorption Spectrophotometer data analysis by One Way Anova shows significant differentiation the amount of dissolved kidney stones calcium in aquadest negative control and 1,2% Calcusol as positive control are 0,3812 ± 0,0027 ppm and  1,4053 ± 0,0051 ppm;   The0,625%, 1,25%, 2,5%, 5% and 10%, Pandanus folium extract could dissolved kidney stones calcium 1,4590 ± 0,0065 ppm, 2,8419 ± 0,0078 ppm, 2,5909 ± 0,0176 ppm, 2,9439 ± ,00740 ppm, 5,4445 ± 0,02562 ppm. The experiment results shows that water extract of Pandanus amarylliflius Roxb.,. Leaves contains flavonoids and could dissolve calcium in kidney stones. The experiment results shows that water extract of Pandanus amarylliflius Roxb., folium contains flavonoids and could dissolve calcium in kidney stones